Boerhavia Diffusa Extract Ameliorates Carbon Tetrachloride Induced Oxidative Stress: an in Vitro Study
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چکیده
Aim: The aim of the present study was to evaluate the effect of Boerhaviadiffusa extract against carbon tetrachloride (CCl4) – induced lipid peroxidation in liver of mice in vitro. Methods: Antioxidant activity of medicinally important plant Boerhaviadiffusawas determined by DPPH – radical scavenging activity. Liver homogenates were prepared and used for various treatments (CCl4 and Boerhaviadiffusa) followed by analysis of lipid peroxidation and protein content by suitable method. Results: Carbon tetrachloride (10 μg/ml) to liver homogenates significantly increases H2O2-induced lipid peroxidation in vitro. An addition of Boerhaviadiffusa aqueous extract (25-150 μg/ml) significantly (p<0.05) reduced CCl4 induced lipid peroxidation in liver homogenates. The effect was concentration-dependent. The result was compared with standard drug Liv. 52. Conclusion: The study confirmed the ameliorative effect of the extract of Boerhaviadiffusaeven higher than the Liv. 52, which may be attributed due to its antioxidative property.
منابع مشابه
An in vivo and in vitro investigation on hepatoprotective effects of Pimpinella anisum seed essential oil and extracts against carbon tetrachloride-induced toxicity
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